Steviol glycosidesrebaudioside (reb) A, C and D have low aqueous solubilities. To improve their aqueous solubilities, inclusion complex of steviol glycosides, reb A, C and D and gamma cyclodextrin were prepared by freeze drying method and further characterized by means of differential scanning calorimetry, Fourier transform infrared spectroscopy and Raman spectroscopy. The effect of gamma cyclodextrin on chemical shifts of the steviol glycosides was also studied in proton NMR experiments as well as in solid state 13C CP/MAS NMR experiments. These results indicated that the steviol glycosides were clearly in inclusion complex formation with the gamma cyclodextrin which also results in solubility enhancement of these steviol glycosides. Phase solubility studies showed that amounts of soluble reb A, C and D increased with increasing amounts of gamma cyclodextrin indicating formation of 1:1 stoichiometric and higher order inclusion complexes.
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机译：甜菊醇糖甙rebaudioside（reb）A，C和D的水溶性低。为了提高其水溶性，通过冷冻干燥法制备了甜菊糖苷，reb A，C和D和γ-环糊精的包合物，并通过差示扫描量热法，傅里叶变换红外光谱和拉曼光谱进一步表征。还在质子NMR实验以及固态13C CP / MAS NMR实验中研究了γ-环糊精对甜菊糖苷化学位移的影响。这些结果表明，甜菊糖苷显然与γ-环糊精形成包合物，这也导致这些甜菊糖苷的溶解度提高。相溶解度研究表明，可溶的Reb A，C和D的含量随γ-环糊精含量的增加而增加，表明形成了化学计量比为1：1的高阶包合物。
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